Direct immobilization of Thermomyces lanuginosus lipase mediated by VKT-peptide for efficient biodiesel production from Jatropha curcas oil
Biodiesel is an environmentally friendly bio-liquid fuel.For the enzymatic production of biodiesel,there is a pressing need for cost-effective and efficient immobilized lipase.In this study,solid-bound peptide VKT-fused Thermomyces lanuginosus lipase(TLL)was directly immobilized on unmodified silica-containing materials to create a novel biocatalyst.Among the silica-based materials(ZSM-5,Na-Y,SAR-100,MCM-41 and SiO2 powder)tested,TLL-VKT immobilized on ZSM-5 zeolite(TLL-VKT@ZSM-5)showed the best immobilization efficiency and maximum loading,as well as exceptional stability in terms of pH,temperature,storage and elution.When used as a biocatalyst at 40℃for 48 hours,TLL-VKT@ZSM-5(1%,w/w,oil)resulted in a biodiesel yield of 93.9%from Jatropha curcas oil.Furthermore,TLL-VKT@ZSM-5 exhibited high reusability,with a biodiesel yield of 71.9%after 7 repeated uses.The immobilization method in this study offers the advantages of simplicity,high efficiency,high stability,and reusability.In the context of enzyme immobilization used in the production of various chemicals,VKT peptides present significant potential.
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