Synthesis and application of coumarin based fluorescent probe for sequential recognition of Cu2+and PO43-
A novel dual-function coumarin based fluorescent probe was prepared from 4-(diethylamino)salicylaldehyde with methyl hydrazinecarboxylate,and its structure was characterized by NMR,FTIR,and HRMS.Fluorescence emission spectra showed that in methanol solution,within 30 seconds,probe L formed a 1∶1 complex L-Cu2+with Cu2+which caused a fluorescence quenching effect with a quenching rate of 100％.It had good specificity for Cu2+and could resist the interference of metal ions such as Ba2+,Li+,Cu+,Al3+,Zn2+,Cs2+,Mn2+,Ca2+,Hg2+,Cd2+,K+,Mg2+,Na+,Co2+,Ag+,Fe3+and Cr2+.And the detection limit was as low as 1.70×10-8mol/L with high sensitivity.Meanwhile,based on the ligand substitution principle,complex L-Cu2+could be used as a fluorescence sensor to achieve fluorescence-enhanced response recognition of PO43-.The process with good selectivity,could resist the interference of common anions(NO2-,CO32-,SO32-,HPO42-,P2O74-Br-,F-,HCO3-,SO42-,C2O42-,Cl-and CH3COO-),and the detection limit was as low as 6.00×10-8mol/L.In addition,probe L was successfully applied in the fields of pharmaceutical chemistry and chemical environmental protection with excellent experimental results.
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