Construction of an efficient microbial community for petroleum hydrocarbon degradation and its remediation performance for oily soil
In order to improve the effectiveness of microbial treatment of oily sludge,this study screened 4 efficient petroleum hydrocarbon degrading bacteria R,J,C and K from oilfield oily sludge,and identified them as Pseudomonas aeruginosa,Candida tropicalis,Rhodococcus erythropolis,and Bacillus subtilis through 16S rDNA.These strains of bacteria could metabolize biosurfactants and had good degradation performance on crude oil.Through antagonistic experiments,surface tension measurements,and analysis of the degradation performance of different components of petroleum hydrocarbons,combined with the principle of complementary advantages,it was ultimately determined to combine strains R,K,J,and C in a ratio of 1∶1∶1∶1 to construct an efficient composite degradation microbial community.The composite microbial community could exert synergistic effects among different strains,further improving the degradation performance of petroleum hydrocarbons.Under the same conditions,the degradation rate of petroleum hydrocarbons reached 91.2％,which was significantly better than that of a single strain.Through field application,it was found that after 60 days of composite microbial community bioaugmentation treatment,the oil content in the soil decreased from the initial 4.97％to 0.34％,meeting the national second class standard for arable land.This indicated that the microbial community had broad application prospects in the remediation of oily soil.
oily soilmicrobial remediationbiodegradationbacterial screeningconstruction of composite microbial communities
万方数据
维普
