Synergistic biodegradation of 2,4-DCP in soil by bovine bone char-based bacterial agent(HD)with earthworms and its impact on microbial communities
2,4-Dichlorophenol(2,4-DCP),a typical chlorophenol compound,was used as the target pollutant.Bovine bone char was used as a carrier to load the HD bacterial strain,thereby producing solid engineered bacterial agents to degrade 2,4-DCP in contaminated soils.The succession and changes of microbial communities in soil and earthworm guts under the stress of 2,4-DCP were revealed using high-throughput sequencing.The results showed that when the bovine bone char,with a particle size range of 160-250μm,was pyrolyzed for 4h,the viable bacteria count was the highest,reaching 2.42×109cfu/g.When the concentration of 2,4-DCP in the soil was 15mg/kg,the effect of solid engineered bacterial agent combined with earthworm remediation group was significantly higher than that of the blank control group and the earthworm-only group.Scanning electron microscopy images showed that strain HD could be stably loaded into the surface voids of bovine bone char,and the surface of the solid engineered bacterial agent showed a porous honeycomb structure.The results of microbial sequencing showed that the input of earthworms and solid engineered bacterial agent could increase the total number and diversity of soil microorganisms.This study aims to provide theoretical and practical references for the development of bioremediation techniques for organic polluted soils.
国家科技期刊平台
NSTL
万方数据
