Quinoline degradation performance of the sequencing batch biofilm reactor enhanced by Pseudomonas sp.strain LV1
Pseudomonas sp.strain LV1,a quinoline-degrading bacterium,was taken as the research object.Firstly,the effects of carbon source on the quinoline degradation performance of strain LV1 were studied,and the results showed that the appropriate addition of sodium acetate could promote quinoline degradation of strain LV1.When the sodium acetate dosage was 1.00g/L,the promotion effect was the most significant.Based on this,a sequencing batch biofilm reactor was successfully started by applying sodium acetate and stepwise increasing quinoline concentration.Then,the strain LV1 was inoculated into the reactor to investigate its effects on the degradation performance of quinoline,and results indicated that the removal rates of quinoline and CODcr in the effluent were stable at 95.70%±1.25%and 86.49%±0.99%,respectively,after the reactor inoculated with strain LV1 was run for 35d.Compared with uninoculated LV1,the removal rate of quinoline and CODcr increased by 46%and 33%,respectively,indicating that strain LV1 could enhance the degradation of quinoline in the reactor.Finally,the microbial community structure before and after reactor strengthening was analyzed through the 16s rDNA high-throughput sequencing technology.After inoculation with LV1,the microbial diversity and microbial community structure of the system were significantly changed.Pseudomonadaceae and Comamonadaceae were the core populations that degraded quinoline pollutants in the start-up stage and the bioaugmentation stages.Among them,the relative abundance of Comamonadaceae belonging to Betaproteobacteria increased significantly during the bioaugmentation stage.According to PICRUSt,the relative abundance of the genes encoding aromatic ring lyase,especially the genes encoding nitrogen heterocyclic lyase,was significantly increased in the start-up stage and the bioaugmentation stages,which were conducive to maintaining or enhancing the degradation performance of quinoline.
quinoline degradationPseudomonas sp.sequencing batch biofilm reactorbioaugmentationmicrobial community structure
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