Intensification of butyric acid fermentation in kitchen waste system
This study investigated the feasibility of butyric acid production from kitchen waste using butyric acid fermenting bacteria,Clostridium tyrobutyricum.The extraction of fermentable sugars from kitchen waste was controlled through various pretreatment methods involving acid,alkali,and temperature adjustments to optimize the hydrolysis of polysaccharides.Under the optimized conditions of 1.5％(mass concentration)sulfuric acid,121 ℃,and 1h,the polysaccharide conversion efficiency reached 62.8％.Following acid pretreatment,the cellulose content in the kitchen waste was enzymatically hydrolyzed more effectively,leading to an enhanced conversion efficiency of polysaccharides.The potential of butyric acid production via Clostridium tyrobutyricum fermentation,augmented by carbon dots,was assessed using the acid-pretreated effluent from kitchen waste as a substrate.Results indicated a 12％increase in butyric acid yield with the addition of carbon dots compared to the control group without them,while the acetic acid content decreased by 27％.Electrochemical analyses demonstrated a 11％increment in the capacitance of Clostridium tyrobutyricum and a 12.6％reduction in resistance upon the introduction of carbon dots.This observation suggested that the inclusion of carbon dots enhanced the electroactivity of the bacterium,potentially accelerating the electron transfer rate within the intracellular Rhodobacter nitrogen fixation(Rnf)complex and elevating the intracellular NADH/NAD+coenzyme ratio.Consequently,this enhancement could facilitate the conversion of acetic acid to butyric acid.This study is anticipated to offer practical methodologies and theoretical foundations for the effective utilization of kitchen waste resources.
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