摘要
利用白及多糖的生物黏附性制备共载黄芪甲苷-黄连素/白及多糖微球(AS-IV-BBR/BSP-Ms),并对其性能进行评价.结果表明,采用乳化交联法制备得到共载AS-IV-BBR/BSP-Ms,微球粒径在30～40 μm,Zeta电位为-21.4 mV,且30 min内溶胀率达54.3％;红外光谱分析、X-射线衍射分析表明,黄芪甲苷和黄连素被包裹于白及多糖微球内部;DSC分析表明,在t=121 ℃时,出现尖锐吸热峰,热稳定性较好;体外抗氧化活性实验表明,该微球DPPH自由基的半数清除浓度(IC50)为8.23 mg/mL.该制备工艺稳定可行,微球性能评价达到预期目标,可为下一步药效学评价提供实验依据.
Abstract
co-Delivery Astragaloside Ⅳ-berberine with Bletilla striata polysaccharide Microspheres(Ms)was prepared by using the bioadhesive property of bletilla striata polysaccharide and their properties were evaluated.The results showed that co-delivery Astragaloside Ⅳ-berberine with bletilla striata polysaccharide microspheres(AS-IV-BBR/BSP-Ms)were prepared by emulsion crosslinking method,the particle size of the drug-carrying microspheres was 30～40 μm,the zeta potential was-21.4 mV,the swelling ratio could reach upwards of 54.3％in 30 min.The infrared spectroscopy and X-ray diffraction analysis showed that astragaloside Ⅳ and berberine were wrapped in the white and polysaccharide microspheres.DSC analysis showed that a sharp endothermic peak appeared at about 121 ℃ with good thermal stability.The in vitro antioxidant activity experiments showed that the DPPH radical scavenging IC50 was 8.23 mg/mL.The preparation process was stable and feasible,and the properties evaluation of microspheres reached the expected goal,which could provide experimental basis for the next pharmacodynamic evaluation.
基金项目
陕西省科技厅科技计划项目青年项目(2023-JC-QN-0822)
2022年度陕西高校青年创新团队科研计划项目(22JP075)
陕西省科技厅自然科学研究项目(2022JM-568)
陕西高校青年创新团队建设项目(陕教[2019]90号)
西安医学院科技创新团队配套(2021TDPT04)
西安医学院科技能力提升项目(2022NLTS066)
维普
万方数据