New extraction technology of flavonoids from seed meal,a by-product of Perilla
The process of extracting flavonoids from Perilla seed by ionic liquid-assisted microwave method was optimized to provide a basis for further utilization of seed meal,a by-product of Perilla.Ionic liquids are added to the extraction solvent to assist in the extraction of flavonoids from Perilla seed meal by microwave method.The optimal extraction condition was optimized,on the basis of the results of single factor experiments,combined with the design of the response surface.Then the extraction rate of total flavonoids was used as an index to determine the optimal conditions of single factors,while the content of caffeic acid,luteolin,rosmarinic acid,quercetin and luteolin was determined by high performance liquid chromatography in Perilla seed meal extracted by different methods.The optimal extraction conditions were microwave power 860 W,V(extract)∶m(raw material)=30 mL/g,V(ethanol)∶V(ionic liquid)=10,t=70 ℃.The actual extraction yield of flavonoids was 3.18％,which was close to the predicted value of 3.176 9％.It can be seen that the extraction rate of ionic liquid method is higher than that of traditional methods,comparing the content of the main components detected by high performance liquid phase.The new method of microwave-assisted ionic liquid extraction of flavonoids from Perilla seed meal is feasible.
万方数据
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