Fluorescent properties and cytotoxicity of chlorogenic acid-based carbon materials
Chlorogenic acid(CA)-derived luminescent carbon material CA-71 was synthesized by one-step solvothermal method.Fluorescence spectroscopy,confocal imaging and in vitro cell viability experiments were conducted to investigate the fluorescence properties and cytotoxicity of CA-71.It was indicated that the fluorescence emission spectrum of CA-71 exhibited excitation-dependence,giving the maximum excitation wavelength at 400 nm and the corresponding emission 492 nm.In confocal imaging experiments on human umbilical vein endothelial cells(HUVEC)in vitro,CA-71 showed membrane permeability and could target mitochondria in HUVEC cells with a high-degree overlap of CA-71 and Mito-Tracker Green,giving a pearson's coefficient 0.98.In addition,the 24 hour-inhibition of CA-71 at 25.00 mg/L on HUVEC cells in vitro was(7.70±0.93)％,which was close to the inhibitory effect(8.24±1.18)％for chlorogenic acid,showing low-cytotoxicity and no significant difference.
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