Determination of Total Flavonoids in Gannan Combretum Alfredii by Ultraviolet-Visible Spectrophotometry
OBJECTIVE:To establish a method for the determination of total flavonoids in Gannan Combretum alfredii.METHODS:The total flavonoids in Gannan Combretum alfredii were extracted by ultrasonic extraction,and the total flavonoids content was determined by ultraviolet-visible spectrophotometry with rutin as the standard reference,and the methodological investigation was also performed.RESULTS:The total flavonoids content showed a good linear relationship with absorbance(508 nm),the linear regression equation was y=8.652x-8.524E-4,R2=0.9986,with good precision,reproducibility and stability,the average addition recovery and RSD was 97.74%,1.84%(n=6),respectivity.CONCLUSIONS:The established ultraviolet-visible spectrophotometry with aluminium nitrate as chromogenic reagent for the determination of total flavonoids in Gannan Combretum alfredii presented in the experiment is rapid,accurate,simple to operate and specific,and can be used as a method for its determination.
NETL
NSTL
万方数据
