淡黑雷松藻低聚糖硫酸酯的制备及抗凝血活性研究
Study on the Preparation and Anticoagulant Activity of oligosaccharides Sulfate from Lessonia Nigrescens
刘浩 1孙彦庆 2邵萌 1李福臣 2李全才1
作者信息
- 1. 青岛海洋生物医药研究院,山东青岛 266071
- 2. 青岛黛优佳生物科技有限公司,山东青岛 266071
- 折叠
摘要
以淡黑雷松藻(Lessonia nigrescens)为原料,采用分步提取和定向改性的方法获得低聚糖硫酸酯ACV1,对其进行了糖醛酸含量、硫酸基含量、相对分子质量、单糖组成理化性质分析,采用傅里叶变换-红外光谱(FT-IR)和核磁共振碳谱(13C-NMR)对其结构进行了表征.体外抗凝血试验结果显示,ACV1可显著延长活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和凝血酶时间(TT)(P<0.05),减少纤维蛋白原(FIB)的生成(P<0.05).体内斑马鱼试验结果显示,ACV1可显著加快斑马鱼的节间血管血流速度(P<0.01).ACV1作为一种天然活性原料具有良好的抗凝血活性,有潜力改善高凝血状态的药品、日用化妆品等功能性产品的开发.
Abstract
In this study,oligosaccharide sulfate ACV1 was obtained from Lessonia nigrescens by step-by-step extraction and directional modification.physicochemical properties of ACV1 were analyzed,including uronic acid content,sulfate content,relative molecular weight,and monosaccharide composition.the structure of ACV1 was characterized using fourier transform Infrared Spectroscopy(FT-IR)and Nuclear magnetic resonance carbon Spectroscopy(13C-NMR).In vitro anticoagulation experiments showed that ACV1 significantly prolonged activated partial thromboplastin time(APTT),prothrombin time(PT),and thrombin time(TT)(P<0.05),and reduced the production of fibrinogen(FIB)(P<0.05).In vivo,zebrafish experiments revealed that ACV1 significantly accelerated the blood flow velocity in the intersegmental vessels of zebrafish(P<0.01).As a natural active ingredient,ACV1 possesses good anticoagulant activity and has the potential to be applied in the development of functional products such as drugs and daily cosmetics for improving hypercoagulable states.
关键词
淡黑雷松藻/低聚糖硫酸酯/理化分析/结构表征/抗凝血试验/斑马鱼试验Key words
lessonia nigrescens/oligosaccharide sulfate/physical and chemical analysis/structural characterization/anticoagulation test/zebrafish experiment引用本文复制引用
出版年
2024
NETL
NSTL
万方数据