Fluorescent dyes for super-resolution imaging of organelles
Super-resolution microscopy has revolutionized the field of cell biology by providing enhanced imaging capabilities that surpass the diffraction limit of conventional light microscopy.In this context,organic small molecule dyes with unique properties such as photostability,ease modification,and tunable fluorescence switching have gained new development opportunities.This review focuses on fluorescent dyes for super-resolution imaging of different cellular organelles and summarizes the design and targeting strategies of currently available super-resolution fluorescent probes.The paper begins by briefly introducing three major super-resolution imaging techniques,including structured illumination microscopy,stimulated emission depletion microscopy,and single-molecule localization microscopy,and their diverse requirements for the performance of fluorescent dyes.It also highlights fluorescent dyes used in super-resolution imaging of mitochondria,lysosomes,cell membranes,lipid droplets,and nucleus over the past five years.Finally,the article discusses the future challenges in this field.
国家科技期刊平台
NSTL
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