Study on encapsulation and properties vitamin A acetate/cyclodextrin
The inclusion complexes of VAA with β-cyclodextrin(β-CD)and its derivatives hydroxypropyl β-cyclodextrin(HP-β-CD)and sulfobutyl etherβ-cyclodextrin(SBE-β-CD)were prepared by freeze-drying method,and the inclusion complexes were characterized by Fourier infrared spectroscopy(FT-IR),nuclear magnetic resonance hydrogen spectroscopy(1H NMR),and scanning electron microscope(SEM),and phase solubility,thermal stability,solubility and molecular binding mode were analyzed.The results of the phase solubility experiments showed that all the three cyclodextrins formed an inclusion ratio of 1∶1 with VAA,among which HP-β-CD had the highest stability constant and was more effective than the other two cyclodextrins in the inclusion of VAA.The experimental results of thermal stability and solubility change tests showed that the thermal stability and water solubility of the three inclusion compounds were substantially improved compared with free VAA,with VAA/HP-β-CD being the best,with the heat loss rate decreasing from 52.6％to 5.2％;and the water solubility increasing from 1.9x10-4to 100 g/100 g.The results of the molecular docking showed that HP-β-CD enlarged the β-CD cavity length so that VAA was completely encapsulated and formed intermolecular hydrogen bonding with VAA,and the inclusion complex formed with VAA had minimal binding energy.The above results indicate that HP-β-CD is an excellent encapsulation carrier for VAA and can effectively improve the application performance of VAA.
vitamin A acetatecyclodextrinstabilitysolubilitymolecular simulation
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