Preparation and biological safety evaluation of surface modified PET reticular fiber scaffold materials
Using ammonia as plasma gas,the surface of synthetic polymer polyethylene terephthalate(PET)scaffold material was treated with low-temperature plasma,and a kind of reticular fiber scaffold material with improved hydrophilicity and biocompatibility was obtained.The material was characterized physically and chemically by XPS photoelectron spectroscopy,water contact angle,electron microscopy,and BET-specific surface area measurement.At the same time,hepatocytes were cultured to measure their biocompatibility,and in vitro cytotoxicity,intradermal reaction,and skin sensitization tests were carried out to evaluate their biological safety.The results showed that after low-temperature plasma treatment,N element was introduced into PET material.Compared with that before treatment,the percentage of O/C elements decreased by 2.05 percentage points,and the percentage of N/C elements increased by 2.10 percentage points.The water contact angle decreased from 123.32° to 30.55°,and the hydrophilicity was significantly improved.The surface roughness of PET material was increased after plasma treatment,and the specific surface area of the material was 0.37 m2/g.Plasma treatment can improve the biocompatibility of PET materials and promote C3A cell adhesion and proliferation.Compared with the untreated group,the treated group showed better biological activity.At the same time,PET-LTPT had good biological safety.MTT cytotoxicity assay in vitro showed that the cell survival rate of the PET-LTPT extract group was 135.82％,higher than that of the negative control group and blank group.It indicated that PET-LTPT material had no cytotoxicity in vitro.The results of the intradermal reaction test showed that the difference between the average score of PET-LTPT in different extraction media and the control group was less than 1.0,and there was no intradermal reaction.The skin sensitization test results showed that the PET-LTPT scaffold material did not produce delayed-type hypersensitivity to guinea pig skin.PET-LTPT is a potential three-dimensional liver tissue culture scaffold material for the bioartificial liver.
NETL
NSTL
万方数据
维普
