As the global number of chemicals continues to increase,the traditional toxicity assessment methods that employed mammalian model are no longer adequate to meet the demands of environmental regulation.Consequently,the toxicity testing methodologies employ in the 21st century have shifted to a new paradigm which is mainly based on high-throughput in vitro testing and the alternative methods in the non-mammalian models.Zebrafish,due to its biological complexity and analytical throughput,is currently an important animal model in high-throughput drug screening and toxicity testing of chemicals.In this article,we reviewed the most important research advances of high-throughput toxicity assessment of chemicals by the zebrafish model.We also summarized the advantages and potential of multiple automated techniques in the workflow of high-throughput analysis by zebrafish.Finally,we proposed several perspectives for future directions of high-throughput toxicity testing by zebrafish.This review will provide systematic supports to researchers to efficiently use zebrafish embryo in safety assessment of chemicals.
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