Treatment of RO concentrate of leachate by salt-tolerant bacteria enhanced biotechnology coupled catalytic ozonation and analysis of microbial characteristics
Treatment of RO concentrate of leachate by salt-tolerant bacteria enhanced biotechnology coupled catalytic ozonation and analysis of microbial characteristics
Aiming at the problems of poor salt tolerance and low treatment efficiency of RO concentrated solution treated by traditional biological treatment process,this study used salt-tolerant bacteria to strengthen the anaerobic/aerobic(A/O)activated sludge coupled catalytic ozonation process to improve the removal of organic matter and total nitrogen.The removal efficiency of pollutants in RO concentrate,the change of organic matter composition before and after treatment,and the differences in microbial characteristics of the three systems of activated sludge,salt-tolerant bacteria and activated sludge/salt-tolerant bacteria were compared.The results showed that the conversion rate of organic nitrogen in anaerobic enhanced system was increased by 42％and 37％,respectively,compared with single anaerobic activated sludge and salt-tolerant bacteria system,and compared with the single aerobic activated sludge and salt-tolerant bacteria system,the total nitrogen removal rate of aerobic enhanced system increased by 37％and 77％.GC-MS analysis showed that some unsaturated bonds were broken after biological and ozone oxidation treatment of raw water organic matter,which improved the degradation of refractory organics.After treatment,the water quality index can reach the Table 2 standard of"Standard for Pollution Control of Domestic Waste Landfill".High-throughput sequencing results showed that Flavobacterium may play a key role in the ammoniation process under high salt conditions in the anaerobic enhanced system;while Truepera and Paracoccus are the key functional bacteria for efficient removal of total nitrogen under high salinity conditions in aerobic enhanced system.The functional gene prediction results showed that the ammoniation gene npr was the key gene of Flavobacterium,which further confirmed the sequencing results.The relative abundances of nap,nar,nir and nor denitrification genes in the aerobic strengthening system were higher than those in the single system,revealing the mechanism of efficient removal of total nitrogen in the strengthening system.
关键词
RO浓液/耐盐菌/生物强化/微生物群落特性
Key words
reverse osmosis concentrate/salt-resistant bacteria/biological enhancement/microbial community characteristics
维普
万方数据