Construction of a reaction-based fluorescent probe and its application in the detection of mercury ions in environmental water
Mercury is extremely harmful to ecosystems and human health due to its toxicity and mobility.Consequently,the development of a convenient,efficient,and rapid analytic method for the detection of Hg2+is of great importance.Herein,Cy-DMTC was designed and synthesized as a reaction-based fluorescent probe by using hemicyanine dye as the fluorescent group and N,N-dimethylthiocarbamoyl as the recognition group.The results of the fluorescence titration investigation demonstrate that Cy-DMTC can specifically recognize Hg2+with high sensitivity and strong anti-interference ability.The fluorescence intensity at 512 nm exhibits a consistent increase as the concentration of Hg2+is raised,resulting in strong green fluorescence and a substantial Stokes shift(156 nm).The fluorescence intensity of Cy-DMTC exhibits an excellent linear correlation with Hg2+concentration in the range of 2.0×10-6-12.5× 10-6 mol·L-1(R2=0.9955),with a detection limit of 8.65× 10-8 mol·L-1.Mechanism studies revealed that Hg2+triggers the hydrolysis of thiocarbonate,thereby enhancing charge transfer fluorescence and making Hg2+detectable both qualitatively and quantitatively.Cy-DMTC has been successfully used to detect Hg2+in real water samples,thus providing an effective method for Hg2+detection.
NETL
NSTL
万方数据
