一种反应型荧光探针的构建及其在环境水汞离子检测中的应用
Construction of a reaction-based fluorescent probe and its application in the detection of mercury ions in environmental water
王倩 1刘永浪 1吴露芳 1张文锦 1左晓玲 2杨武德 1吴翀1
作者信息
- 1. 贵州中医药大学药学院,贵阳,550025
- 2. 贵州民族大学材料科学与工程学院,贵阳,550025
- 折叠
摘要
汞因具有高毒性、迁移性等特点对环境及人体产生巨大的伤害.因此,建立便捷、高效、快速检测Hg2+的分析方法具有重要意义.本文以半花菁染料为荧光基团,N,N-二甲氨基硫代甲酸酯为识别基团,设计合成了一种反应型荧光探针Cy-DMTC.荧光光谱滴定分析显示探针Cy-DMTC能够特异性识别Hg2+,且灵敏度高、抗干扰能力强.随着Hg2+浓度增加,其512 nm处荧光强度不断增强且呈现出强绿色荧光,同时产生了显著的stokes位移(156 nm).探针的荧光强度与Hg2+浓度在2.0×10-6-12.5×10-6 mol·L-1范围内呈现良好的线性相关(R2=0.9955),检出限为8.65×10-8mol·L-1.机理研究证实由于Hg2+诱导硫代甲酸酯发生水解反应,导致电荷转移荧光增强,从而实现对Hg2+的定性定量检测.探针Cy-DMTC已被成功应用于实际水样中Hg2+的检测,可为Hg2+的检测提供了一种高效的解决方案.
Abstract
Mercury is extremely harmful to ecosystems and human health due to its toxicity and mobility.Consequently,the development of a convenient,efficient,and rapid analytic method for the detection of Hg2+is of great importance.Herein,Cy-DMTC was designed and synthesized as a reaction-based fluorescent probe by using hemicyanine dye as the fluorescent group and N,N-dimethylthiocarbamoyl as the recognition group.The results of the fluorescence titration investigation demonstrate that Cy-DMTC can specifically recognize Hg2+with high sensitivity and strong anti-interference ability.The fluorescence intensity at 512 nm exhibits a consistent increase as the concentration of Hg2+is raised,resulting in strong green fluorescence and a substantial Stokes shift(156 nm).The fluorescence intensity of Cy-DMTC exhibits an excellent linear correlation with Hg2+concentration in the range of 2.0×10-6-12.5× 10-6 mol·L-1(R2=0.9955),with a detection limit of 8.65× 10-8 mol·L-1.Mechanism studies revealed that Hg2+triggers the hydrolysis of thiocarbonate,thereby enhancing charge transfer fluorescence and making Hg2+detectable both qualitatively and quantitatively.Cy-DMTC has been successfully used to detect Hg2+in real water samples,thus providing an effective method for Hg2+detection.
关键词
荧光探针/半花菁/N/N-二甲氨基硫代甲酸酯/汞离子/反应型探针Key words
fluorescent probe/hemicyanine/N,N-dimethylthiocarbamoyl/mercury ion/reaction-based probe引用本文复制引用
基金项目
国家自然科学基金(81903799)
贵州省科技支撑计划(黔科合支撑[2020]4Y107)
贵州省教育厅自然科学研究项目(黔教合 KY 字[2021]068)
出版年
2024
NETL
NSTL
万方数据