Determination of dimethyl sulfide and methyl mercaptan in blood by headspace gas chromatography
Methyl sulfide and methyl mercaptan,as the main metabolites of hydrogen sulfide after entering the human body,have certain marking effects.A headspace gas chromatographic method was developed for the detection of methyl sulfide and methyl mercaptan in blood.A flame photometric detector(FPD)with high sensitivity for sulfur-containing compounds was used,and it was found that the addition of 0.15 g sodium chloride to 0.5 mL of blood sample resulted in improved detection efficiency.The standard curve range of this method was 0.01-2.00 μg·mL-1,the correlation coefficient(R2)was 0.997,the limit of detection was 0.003 μg·mL-1,and the limit of quantification was 0.01 μg·mL-1.Although methyl mercaptan is not suitable for quantitative analysis due to its low boiling point,its qualitative detection can also provide a basis for hydrogen sulfide poisoning.The method developed in this study can be directly applied to the determination of trace amounts of methyl mercaptan and methyl mercaptan in blood,thus providing a basis for the identification of hydrogen sulfide poisoning cases.
gas chromatographymethyl sulfidemethanethiolhydrogen sulfide
NETL
NSTL
万方数据
