挺水植物菖蒲根区沉积物细菌群落结构和功能特征分析
The structural and functional characteristics of the sedimentary bacterial community in the root area of Acorus calamus
陶苗苗 1李亚楠 1宋海涯 1李维斌 1周晓红 1许小红2
作者信息
- 1. 江苏大学环境与安全工程学院,镇江,212013
- 2. 江苏大学环境与安全工程学院,镇江,212013;江苏大学应急管理学院,镇江,212013
- 折叠
摘要
挺水植物根区是湖泊等淡水生态系统物质循环最为活跃的微生态区域之一.为了进一步明确挺水植物在不同生长阶段对根区沉积物微生物的影响,本论文以挺水植物菖蒲(Acorus calamus)为研究对象,构建室外盆栽实验系统,采集实验第35、105和175天时菖蒲根区及无植物对照系统沉积物样品,研究菖蒲生长发育对根区沉积物细菌群落及功能的影响.结果表明,菖蒲根区沉积物共检测OTUs数量11659个,无植物的沉积物样品获得OTUs数量为10337个.基于OTUs结果的分析发现,菖蒲根区沉积物Shannon、Sobs、ACE和Chao指数平均值表现为CA＞CK;相比于对照组而言,菖蒲根区独有的OTUs数量略高,意味着菖蒲生长可能会丰富根区沉积物细菌的物种组成,尤其是对于细菌群落中相对丰度占比较小的物种产生显著影响;通过FAPROTAX对菖蒲根区及对照系统沉积物细菌代谢及生态功能进行注释,共得到67种细菌群落功能类型,以化能异养、需氧化能异养、发酵、固氮作用、光合自养、亚硝酸盐还原功能等类群为主,菖蒲的生长将提高氮循环功能类群的相对丰度从而对沉积物氮循环过程产生一定的影响,且这种影响将随菖蒲的不同生长阶段而有所变化.沉积物理化参数是驱动菖蒲根区细菌群落的关键因子.
Abstract
The root zone of emergent plants is one of the most active microecological regions for nutrients biogeochemical cycles in freshwater ecosystems.To further clarify the influence of different growth stages of emergent plants on the bacterial community and function in their root zone,Acorus calamus was chosen and used to construct the outdoor experiment system in this study.The sediments samples were collected from both Acorus calamus'root zone and the control system(non-plant sediment)on the 35,105 and 175 days,respectively.A total of 11659 and 10337 OTUs were detected in Acorus calamus and control groups,respectively.The average values of Shannon,Sobs,ACE and Chao indexes were CA＞CK.The number of unique OTUs in Acorus calamus root zone sediments was higher than in the non-plant control system.This suggests that plant growth may enrich the bacteria composition in their root zone,especially for the lower relative abundance species in the bacterial community.A total of 67 functional types of bacteria were predicted by FAPROTAX from the Acorus calamus root zone sediment and non-plant sediment.The main functional groups are chemoheterotrophic,aerobic heterotrophic,fermentation,nitrogen fixation,photosynthetic autotrophic,and nitrite reduction.The growth of Acorus calamus has the potential to increase the relative abundance of nitrogen cycling functional groups and have a certain impact on the nitrogen cycling process in sediment.This influence will vary with different growth stages of Acorus calamus.The bacterial community was influenced by the physicochemical parameters of the sediment in the root zone of Acorus calamus.
关键词
菖蒲/根区/沉积物/细菌群落/功能预测Key words
Acorus calamus/root zone/sediment/bacterial community/function引用本文复制引用
基金项目
国家自然科学基金(31971472)
西藏自治区重点研发计划(XZ202301ZY0002N)
出版年
2024
NETL
NSTL
万方数据