Fully automated on-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry for rapid determination of trace methyl parathion in surface water
A method for rapid and accurate determination of trace methyl parathion in surface water by Fully automated on-line solid phase extraction coupled with liquid chromatography tandem mass spectrometry was established.The water samples were enriched online,separated on a chromatographic column with gradient elution,and quantified by internal standard method using electrospray ionization source in positive ions multiple reaction monitoring mode.By optimizing the ion source parameters,changing aqueous phase eluent,organic phase eluent,and initial ratio of eluent,the sensitivity of methyl parathion was significantly improved,and the peak shape got better.The method showed good linearity in the mass concentration range of 5-1000 ng·L-1(Correlation coefficient(R2)≥0.998).Through standard addition experiments,The results showed that the limit of detection of the method was 4 ng·L-1 and the limit of quantification was 16 ng·L-1.The average recovery at 3 addition concentrations of 40,100,and 500 ng·L-1 was 90.1%—97.8%,with a relative standard deviation(RSD)of 1.7%—3.5%.The method was applied to the determination of actual surface water samples,and no trace of methyl parathion was detected.The method is convenient and efficient,and can achieve high-throughput determination of trace methyl parathion in surface water.
Fully automated on-line solid phase extractionliquid chromatographytandem mass spectrometrymethyl parathion
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