The interference factors were analyzed based on the principle of titration and instrumental determination of sugar
The High-performance liquid chromatography,ion chromatography and titration methods were used for the determination of fructose,glucose,sucrose,maltose and lactose in 18 kinds of food,such as milk powder,soybean and peanut.It was found that there was no significant difference between the results of ion chromatography and High-performance liquid chromatography,but there was significant difference between the results of titration and chromatography.Based on the principle of titrimetric determination of reducing sugar and non-reducing sugar,a verification experiment was designed to analyze the interference factors,the results showed that food samples containing non-reducing sugars such as raffinose,stachyose,galactooligosaccharides,fructose,Glucan and resistant dextrin would interfere with the titration results of sucrose;Reducing sugars such as fructose,glucose,maltose and galactooligosaccharides may interfere with the titration results of lactose.
ion chromatographyhigh-performance liquid chromatographytitrationreducing sugarsnon-reducing sugarsinterference