首页|基于滴定法与色谱法测定糖的干扰因素分析

基于滴定法与色谱法测定糖的干扰因素分析

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采用高效液相色谱法、离子色谱法和滴定法对乳粉、黄豆和花生等 18种含有果糖、葡萄糖、蔗糖、麦芽糖、乳糖的食品基质样品进行检测,并对各方法的检测数据进行总体均值检验分析,发现离子色法和液相色谱法之间的检测结果无显著性差异,而滴定法与色谱法部分基质的差异显著。基于滴定法测定还原糖与非还原糖的原理,设计验证实验进行干扰因素分析,结果表明,含有棉子糖、水苏糖、低聚半乳糖、果聚糖、聚葡萄糖和抗性糊精的等非还原性糖的食品样品会干扰蔗糖的滴定结果;果糖、葡萄糖、麦芽糖和低聚半乳糖等还原性糖会干扰乳糖的滴定结果。
The interference factors were analyzed based on the principle of titration and instrumental determination of sugar
The High-performance liquid chromatography,ion chromatography and titration methods were used for the determination of fructose,glucose,sucrose,maltose and lactose in 18 kinds of food,such as milk powder,soybean and peanut.It was found that there was no significant difference between the results of ion chromatography and High-performance liquid chromatography,but there was significant difference between the results of titration and chromatography.Based on the principle of titrimetric determination of reducing sugar and non-reducing sugar,a verification experiment was designed to analyze the interference factors,the results showed that food samples containing non-reducing sugars such as raffinose,stachyose,galactooligosaccharides,fructose,Glucan and resistant dextrin would interfere with the titration results of sucrose;Reducing sugars such as fructose,glucose,maltose and galactooligosaccharides may interfere with the titration results of lactose.

ion chromatographyhigh-performance liquid chromatographytitrationreducing sugarsnon-reducing sugarsinterference

林立、孙海波、邱云、刘莉、齐云肖、杨彦丽、曹瑀

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北京市食品检验研究院(北京市食品安全监控与风险评估中心),北京,100085

国家市场监管重点实验室(食品安全重大综合保障关键技术),北京,100085

离子色谱法 高效液相色谱法 滴定法 还原糖 非还原糖 干扰

国家市场监管总局科技计划项目

2022MK008

2024

环境化学
中国科学院生态环境研究中心

环境化学

CSTPCD北大核心
影响因子:1.049
ISSN:0254-6108
年,卷(期):2024.43(7)
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