摘要
阿特拉津是一种广泛使用的除草剂,同时也是一种内分泌干扰物,在环境水体中被频繁检出,然而目前还缺乏有关阿特拉津对鱼类内分泌干扰效应的全面了解.本文研究了环境相关浓度暴露下阿特拉津对斑马鱼幼鱼不同内分泌激素系统中22种激素的影响,并在激素水平、内分泌激素系统水平、综合内分泌干扰指数水平进行了分析.结果表明,阿特拉津暴露能够引起斑马鱼体内11-脱氧皮质醇(11DF)、皮质酮(COR)、醛固酮(ALDO)、雌酮(E1)、雌二醇(E2)、雄烯二酮(AD)、睾酮(T)、雄酮(ADT)、褪黑素(MT)、甲状腺素(T4)、前列腺素D2(PGD2)、前列腺素E2(PGE2)、前列腺素F1α(PGF1α)、血栓素B2(TXB2)的含量升高,和导致孕酮(P)、孕烯醇酮(PREG)、17α羟基孕酮(OHP)的含量降低.在3个暴露浓度(1、10、100 μg·L-1)中,低浓度的阿特拉津对斑马鱼性激素系统、甲状腺激素系统、松果体激素系统和内分泌细胞激素系统的干扰作用更加明显,同时也具有更强的综合内分泌干扰效应.
Abstract
Atrazine is a widely used herbicide and also an endocrine disruptor that is frequently detected in aquatic environment.However,the comprehensive profile of endocrine disruption of atrazine has been not revealed in fish up to date.The effects of atrazine on 22 hormones of various endocrine hormone systems were investigated in larval zebrafish at environmentally relevant concentrations,and assessed at the levels of hormone,endocrine hormone system and comprehensive index in the study.The results indicated that exposure to atrazine increased the levels of 11-deoxycortisol(11DF),corticosterone(COR),aldosterone(ALDO),estrone(E1),estradiol(E2),androstenedione(AD),testosterone(T),androstenedione(ADT),melatonin(MT),thyroxine(T4),prostaglandin D2(PGD2),prostaglandin E2(PGE2),prostaglandin F1α(PGF1α)and thromboxane B2(TXB2),and decreased the levels of progesterone(P),pregnenolone(PREG),and 17α-hydroxyprogesterone(OHP).Among the three exposure concentrations(1,10 and 100 μg·L-1),the low concentration of atrazine induced stronger effects on the sex hormone system,thyroid hormone system,pineal hormone system and endocrine cell hormone system,and meanwhile posed a greatest comprehensive endocrine disruption in zebrafish.
基金项目
科技部重点研发计划(2022YFC3703204)
NETL
NSTL
万方数据