HNO3-H2O2消解体系对植物中磷和钾元素的快速定量分析
Rapid quantification analysis of plant phosphorus and potassium using HNO3-H2O2 digestion system
熊满艳 1王美娥 1王巧环 1宋祥梅1
作者信息
- 1. 中国科学院生态环境研究中心,城市与区域国家重点实验室,北京,100085
- 折叠
摘要
为研究不同酸溶消解体系对植物样品中磷、钾测定的影响,分别采用H2SO4-H2O2、HNO3-HC1O4、HNO3-H2O2和王水-H2O2等4种酸体系对灌木枝叶标准物质(GBW07603)进行酸溶消解处理,使用电感耦合等离子体发射光谱仪(ICP-OES)测定其中磷、钾的含量,筛选最优的酸溶消解体系.结果表明,HNO3-H2O2消解法对植物样品消解彻底且消解后溶液澄清,测定结果的准确度和精密度最高,安全性好,是最佳消解体系.加酸量±10％对HNO3-H2O2消解法测定磷、钾元素含量结果没有显著影响,但消解温度对其影响较大,最适消解温度为150-180 ℃.在最优条件下,磷的检出限和测定限分别为0.004 g·kg-1和0.018 g·kg-1;钾的检出限和测定限分别为0.06 g·kg-1和0.25 g·kg-1;对8种不同植物标准物质中磷和钾测定值均在标准值范围内,且相对标准偏差和相对误差都小于±5％;对4种不同植物标准物质的加标回收率在91％—106％之间.因此,HNO3-H202消解结合ICP-OES测定方法能满足不同植物的测定要求,可用于大批量样品的快速检测分析.
Abstract
To study effects of different acid digestion systems on plant phosphorus and potassium determination,four acid systems,including H2SO4-H2O2,HNO3-HClO4,HNO3-H2O2 and aqua regia-H2O2,were selected to digest certified reference materials of shrub branches and leaves(GBW07603).Phosphorus and potassium in digested extracts were determined using an inductively coupled plasma emission spectrometer(ICP-OES)by which the optimal acid digestion system was consequently chosen based on accuracy and precision.It was observed that HNO3-H2O2 system digested plant samples completely and the digested extracts were clear.Results of ICP-OES analysis showed the highest accuracy and precision.With the safety property of HNO3-H2O2,it could be concluded that it is optimal.The difference in concentrations of phosphorus and potassium determined between treatments with acid addition ranged±10％was insignificant.However,the digestion temperature had significant impact on the determined concentrations.The optimal digestion temperature was 150-180 ℃.Under optimal digestion conditions,the detection and determination limits of phosphorus were 0.004 g·kg-1 and 0.018 g·kg-1,respectively,while 0.06 g·kg-1 and 0.25 g·kg-1 for potassium,respectively.Determination values for 8 various plant reference materials were all within the standard range,with relative bias and errors less than±5％.The recovery values of added standards for 4 various plant reference materials ranged 91％—106％.Therefore,HNO3-H2O2 combining with ICP-OES determination can satisfy the analysis of phosphorus and potassium in various plants and fits for quick quantification of samples with large quantities.
关键词
电热板消解法/酸溶消解体系/磷/钾/1CP-OESKey words
electrothermal plate decomposition method/acid decomposition system/phosphorus/potassium/ICP-OES引用本文复制引用
基金项目
国家重点研发计划项目(2021YFC1809103)
出版年
2024
NETL
NSTL
万方数据