基于碳量子点共振能量转移荧光猝灭法检测苦味酸
Detection of picric acid by fluorescence resonance energy transfer quenching method on carbon nanodots
邓祥 1黄小梅 1邓子禾 2龚安界 1赵华利3
作者信息
- 1. 四川文理学院化学化工学院,达州,635000;乡村低成本环境治理技术四川省高校重点实验室,达州,635000
- 2. 石河子大学化学化工学院,石河子,832000
- 3. 四川文理学院化学化工学院,达州,635000
- 折叠
摘要
苦味酸(PA)作为一种高爆物和环境污染物,近年来引起了广泛的关注.本研究提出了一种新型荧光碳量子点(C-dots)探针,可用于快速、高选择性和高灵敏度的PA检测.探针采用中药材川木香为碳源,通过水热法一步合成.在荧光碳量子点溶液中加入PA,通过荧光共振能量转移机理,碳量子点的荧光被有效猝灭.结果表明,碳量子点最大激发波长为360 nm,最大荧光发射波长为415 nm,荧光量子产率为28.4％.方法检测PA响应时间2 min,线性范围0.2-800 μmol·L-1,检测限(LOD)63 nmol·L-1,实际水样中加标回收实验,回收率为98.8％—101.7％,相对标准偏差(RSD)为1.2％—2.7％.
Abstract
Picric acid(PA),as a kind of high explosive and environmental pollutant,has attracted wide attention in recent years.This study presents a novel fluorescent carbon nanodots(C-dots)probe that can be used for rapid,high selectivity and high sensitivity PA detection.The probe is synthesized by hydrothermal method in one step with the carbon source of the traditional Chinese medicinal material Radix Vladimiriae.When PA is added to the fluorescent C-dots solution,the fluorescence of C-dots is effectively quenched by the fluorescence resonance energy transfer(FRET)mechanism.The experimental results show that the maximum excitation wavelength of C-dots is 360 nm,the maximum fluorescence emission wavelength is 415 nm,and the fluorescence quantum yield is 28.4％.This method detects the response time of PA is 2 min,the linear range is 0.2-800 μmol·L-1,the detection limit(LOD)is 63 nmol·L-1,the spike-and-recovery experiments in actual water samples,the recovery rate is 98.8％—101.7％,and the relative standard deviation(RSD)is 1.2％—2.7％.
关键词
中药材川木香/荧光共振能量转移(FRET)/碳量子点(C-dots)/苦味酸(PA)Key words
traditional Chinese medicinal material Radix Vladimiriae/fluorescence resonance energy transfer(FRET)/carbon nanodots(C-dots)/picric acid(PA)引用本文复制引用
基金项目
四川省自然科学基金(2019YJ0307)
乡村低成本环境治理技术四川省高校重点实验室(XCH2022ZB-05)
达州市自然科学基金(18YYJC0002)
四川省大学生创新创业训练计划(202210644017)
出版年
2024
NETL
NSTL
万方数据