Compared with solid microbial inoculants,liquid composite microbial inoculants have the advantages of simple preparation method,easy storage,convenient transportation and short start-up time,which can quickly start the reactor and improve practical efficiency.The novel liquid composite microbial inoculants are prepared with the aid of the technical means in the fields of food preservation in this study.It would provide important references for its large-scale production and practical application.The influence of thickeners,emulsifiers and antioxidants on the preservation efficiency of microbial inoculants is investigated and the response surface optimization is conducted.The optimal conditions for preparing liquid composite microbial inoculants are 0.401％arabic gum,0.090％Tween-20 and 0.042％ascorbic acid.The results of effectiveness verification and stability evaluation indicate that ascorbic acid has a significant impact on the enzyme activities of cellulase and dehydrogenase.After 30 days of storage under these conditions,the microorganism survival rate is 63.9％.The number of viable bacteria is 1.86 × 109 CFU·mL-1,a mould count is 1.4 ×105 CFU·mL-1 and a mixed bacterial rate is 0.022％,It meets the technical indicator requirements of China's"Microbial inoculants in agriculture"(GB 20287-2006).This study explores the influence of technical means in the fields of food storage on preserving liquid composite microbial inoculants,and provides a novel method for the preparation of liquid microbial inoculants.
维普
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