摘要
硒是人体必需微量营养元素,具有免疫调节、抗氧化、抗癌等生物学功能.从富硒食品中获取无机硒,是一种安全、健康且有效的补充硒元素的方式.为了解富硒食品中无机硒含量及其对HCT116结肠癌细胞凋亡、迁移、侵袭的影响及机制,采用高效液相色谱-电感耦合等离子体质谱联用(HPLC-ICP-MS)测定富硒食品中无机硒的含量,并以一定浓度的亚硒酸钠处理HCT116结肠癌细胞48h,测定细胞活力、凋亡、迁移、侵袭能力及相关蛋白表达.实验结果表明富硒食品中仅检测出四价硒,且四价硒亚硒酸钠能降低HCT116细胞活力、提高细胞凋亡率、抑制细胞迁移及侵袭能力,下调Bcl-2、MMP-2、MMP-9、intergrin αVβ3、PI3K、p-AKT 及 p-mTOR 蛋白表达,上调 Bax、cleaved caspase 3 及 E-cadherin蛋白表达,差异均具有统计学意义(P＜0.05).从而说明亚硒酸钠对结肠癌的抑制作用,可能是通过阻断PI3K/AKT/mTOR信号通路实现的,为富硒食品功能的深入研究、进一步开发及临床研究提供数据支撑,也为结肠癌的临床治疗的研究提供新的策略和理论参考.
Abstract
Selenium is an essential micronutrient element for the human body,which has biological functions such as immune regulation,antioxidant,and anti-cancer.Inorganic selenium obtaining from Se-enriched foods is a safe,healthy,and effective way to supplement selenium elements.The objective of this study was to explore the inorganic selenium content in Se-enriched foods as well as its mechanisms and effect on apoptosis,migration,and invasion of HCT1116 colon cancer cells.Inorganic selenium content in Se-enriched foods was detected by high performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS).HCT116 colon cancer cells were cultured with a certain concentration of sodium selenite for 48 hours.Cell viability,apoptosis,migration,invasion ability,and related protein expression was measured.The results showed that only tetravalent selenium was detected in Se-enriched foods.Sodium selenite could reduce HCT116 cell viablity,increase cell apoptosis rate,inhibit cell migration and invasion ability,down-regulate the expression of Bcl-2,MMP-2,MMP-9,intergrin αVβ3,PI3K,p-AKT,and p-mTOR,and up-regulate the expression of Bax,cleaved caspase 3,and E-cadherin.The differences were statistically significant(all P＜0.05).The results suggest that the inhibitory effect of sodium selenite on colon cancer may be achieved by blocking the PI3K/AKT/mTOR signaling pathway.So it provides data support for the in-depth study,further development,and clinical research of Se-enriched foods,as well as new strategies and theoretical references for the clinical treatment of colon cancer.
维普
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