Patterns of Bacterial Community Through Soil Depth Profiles and Its Influencing Factors Under Betula albosinensis Burkill in the Xinjiashan Forest Region of Qinling Mountains
In this study, vertical changes in bacterial α-diversity and community composition were investigated at four soil depths(0-10, 10-20, 20-40 and 40-60 cm) in Betula albosinensis Burkill forest of Qinling Mountains by sequencing of the 16S rDNA regions using Illumina MiSeq high-throughput technology. The results showed that the decreases of OTUs, Chao1 and Shannon were numerical but not significant, and the highest values of 1688, 2314 and 8. 66 were obtained in 0-10 cm, respectively. At the phylum level, Acidobacteria and Proteobacteria were the most dominant bacteria in four soil layers. At the genus level, Gp4, Gp6 and Gp16 were the most dominant bacteria. The relative abundance of Acidobacteria in 40-60 cm soil depth(62. 88% ) was higher than those in other soil depths. Proteobacteria in 0-10 cm(23. 62% ) was more abundant than that in 40-60 cm. The relative abundance of Acidobacteria was significantly correlated with the total N, soil organic carbon, C/ N, and soil dissolved organic carbon. Soil water content, soil organic matter and soil dissolved organic carbon were the key factors affecting soil Proteobacteria. RDA sequencing results showed that soil dissolved organic carbon was the key factor contributing to the bacteria community abundance. The results demonstrated that there are plenty of bacterial distribution in all four soil layers, which provides a fundamental basis for vertical soil bacterial community diversity, and possesses very important research value in biogeochemical cycling.
NETL
NSTL
万方数据
维普
