首页|海洋沉积物中硫酸盐还原菌和硫氧化菌群落分析方法的比较

海洋沉积物中硫酸盐还原菌和硫氧化菌群落分析方法的比较

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硫酸盐还原菌(sulfate-reducing prokaryotes,SRP)和硫氧化菌(sulfur-oxidizing prokaryotes,SOP)在硫的生物地球化学循环中发挥着极为重要的作用.本文以SRP作为高丰富度高多样性菌群代表,针对于SRP所共有的异化型亚硫酸盐还原酶(dissimilatory sulphite reductase,DSR)中的 β 亚基基因(dsrB),通过克隆文库技术、454高通量测序技术和Illumina高通量测序技术对其群落特征进行比较分析.结果表明,Illumina高通量测序技术优于454高通量测序技术和克隆文库技术,特别在低丰度物种的检测方面,Illumina高通量测序技术具有明显优势.以SOP soxB基因(~750 bp)作为较长基因片段的代表,分别采用454高通量测序技术和Illumina单端高通量测序技术对SOP群落组成和多样性信息进行比较分析,结果表明,454高通量测序技术在读长上的优势并未体现出来,而Illumina单端高通量测序技术优于454高通量测序技术.
Analysis of Sulfate-Reducing and Sulfur-Oxidizing Prokaryote Community Structures in Marine Sediments with Different Sequencing Technologies
Sulfate-reducing prokaryotes (SRP) and sulfur-oxidizing prokaryotes (SOP) play vital roles in the sulfur cycle. The SRP community was used to represent a microbial community with high richness and diversity. The 454 pyrosequencing, Illumina high-throughput sequencing, and traditional clone library methods that target the dissimilatory sulfite reductase β subunit gene ( dsrB), which encodes a key enzyme in the sulfate reduction pathway, were used to compare the differences in SRP community characteristics. Comparative analyses suggested that Illumina high-throughput sequencing was a more appropriate method for SRP (high richness and diversity) community studies. The SOP soxB gene ( ~ 750 bp) was used as a representative of the long-sequence segment. The 454 pyrosequencing and Illumina high-throughput sequencing methods were used to compare the differences in SOP community characteristics. The results revealed that 454 pyrosequencing did not reflect its advantage of a longer read length; whereas, the Illumina high-throughput sequencing with more numerous and shorter sequence reads was more suitable when the soxB gene was used to investigate the community composition and diversity of SOP.

sulfate-reducing prokaryotes ( SRP)sulfur-oxidizing prokaryotes ( SOP)high-throughput sequencingclone librarysediments

张玉、米铁柱、甄毓、陈烨、付璐璐、王勋功

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中国海洋大学环境科学与工程学院, 青岛 266100

海洋环境与生态教育部重点实验室, 青岛 266100

青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 青岛 266071

中国海洋大学海洋生命学院, 青岛 266003

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硫酸盐还原菌 硫氧化菌 高通量测序 克隆文库 沉积物

国家自然科学基金国家自然科学基金中国科学院海洋生态与环境科学重点实验室项目青岛海洋科学与技术国家实验室海洋生态与环境科学开放基金

4152106441620104001KLMEES201601

2018

10.13227/j.hjkx.201705054

环境科学
中国科学院生态环境研究中心

环境科学

CSTPCDCSCD北大核心EI
影响因子：1.913
ISSN：0250-3301
年,卷(期)：2018.39(1)
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