The composition and structure signatures of four dissolved organic matter (DOM) were analyzed with Ultraviolet-visible(UV-Vis) and threedimensional fluorescence spectroscopy characteristics,and their effects on the adsorption ofβ-HCH in the soil were studied.The results showed that,DOM extracted from different soil has different composition and effect on the adsorption ofβ-HCH in the soil.According to the spectral characteristics,aromaticity and hydrphobicity of DOM were in order of nutritional soil>sample A>sludge>sample B.Under the condition of removing DOM,adsorption of β-HCH in the soil,and the adsorption capacity was related with the hydrophily and hydrphobicity of DOM.The adsorption ability ofβ-HCH in the soil can be promoted by exogenous DOM.Because of the different component and hydrophobicity of DOM,the effects on the adsorption ofβ-HCH in the soil were different,the adsorption ofβ-HCH in the soil was in order of sludge>nutrition soil>sample B.