首页|旱地土壤反硝化厌氧甲烷氧化菌的垂向分布特性与活性分析

旱地土壤反硝化厌氧甲烷氧化菌的垂向分布特性与活性分析

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在厌氧条件下，以亚硝酸盐作为电子受体将甲烷氧化的反硝化厌氧甲烷氧化反应(nitrite-dependent anaerobic methane-oxidizing，n-damo)的发现，彻底颠覆了人们对甲烷循环的传统理解。通过分子生物学方法及13C和15N稳定同位素示踪技术，对河北省北澧河附近的旱地农田土壤(0～1 m)中n-damo菌的群落结构、丰度和活性进行了研究，深入探究了n-damo菌的亚硝酸盐底物来源。结果显示，n-damo菌更多存在于旱地浅层土壤中，并且随季节变化分布在不同深度的土壤中。针对其pmoA基因的系统发育分析显示，旱地土壤中n-damo菌的群落结构具有明显的空间异质性，来自土壤0～ 20 cm和40～ 60 cm土层的序列完全分开，处于系统发育树不同分枝。针对其16S rRNA基因的实时荧光定量PCR结果显示，n-damo菌丰度随土壤深度的增加而降低，夏季丰度(1。44× 104～ 1。02× 105 copies·g-1)低于冬季(3。66× 104～ 2。67× 105 copies· g-1)。在浅层土壤(0～20 cm)中，硝化反应和反硝化反应共同为n-damo菌提供亚硝酸盐底物来源;而在深层土壤(60～ 80 cm)中，亚硝酸盐底物主要来源于硝化反应。n-damo菌的活性(0。18 nmol· g-1· d-1，以CO2计)只能在夏季表层土壤(0～20 cm)中检测到，其余深度均未检测到其活性。在旱地农田土壤中，反硝化厌氧甲烷氧化菌对农田碳循环的影响可能不大。
Vertical distribution characteristics and activity analysis of nitrite-dependent anaerobic methane oxidation in an agricultural upland soil core
The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) with nitrite as the electron acceptor has changed the understanding about methane oxidation.In this study,the unique distribution characteristics and activity of n-damo bacteria were investigated in upland soil cores (1 m depth) from an agricultural upland at the Beili River in Hebei Province,China.Meanwhile,nitrite source for n-damo bacteria was also studied.Evidence was obtained using molecular biology methods and isotope-tracing technique.Results showed that n-damo bacteria were detected only in the shallow upland soil and distributed at various depths in different seasons.Phylogenetic analysis ofpmoA genes showed apparent spatial heterogeneity of n-damo bacteria,and the sequences from 0～20 cm and 40～60 cm were completely separated in different branches of the phylogenetic tree.The abundance of n-damo bacteria decreased with soil depths,and the abundance in summer (1.44× 104～ 1.02×105 copies·g-1) was lower that in winter (3.66× 104 ～2.67×105 copies· g-1).In this study,we found that the NO~ source for n-damo process came from both nitrification and denitrification processes in surface soils (0 ～ 20 cm),while in deep soils (60 ～ 80 cm) it mainly came from the nitrification process.Moreover,the n-damo activity (0.18 nmol CO2· g-1· h-1) was only observed in surface soil (0～20 cm),and activity at other depths could not be detected.The influence of n-damo bacteria in the agricultural upland soil may be limited.

nitrite-dependent anaerobic methane-oxidizing(n-damo)agricultural upland soiabundanceactivity

周利、李怡潇、庄林杰、刘璐、张坤、祝贵兵

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青岛理工大学环境与市政工程学院,青岛266033

中国科学院生态环境研究中心饮用水科学与技术重点实验室,北京100085

反硝化厌氧甲烷氧化菌 旱地小麦田 丰度 活性

国家自然科学基金国家自然科学基金中国科学院战略性先导科技专项中国科学院青藏高原环境变化与地表过程重点实验室开放基金环境模拟与污染控制国家重点联合实验室专项经费项目中国科学院饮用水科学与技术重点实验室2015年度自由研究课题洪堡奖学金中国科学院青年创新促进会资助项目

4132201221277156XDB15020303TEL20130414L01ESPC15Z07KLDWST1152633

2017

10.13671/j.hjkxxb.2016.0315

环境科学学报
中国科学院生态环境研究中心

环境科学学报

CSTPCDCSCD北大核心
影响因子：1.645
ISSN：0253-2468
年,卷(期)：2017.37(2)
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