In order to search for efficient PAH degrading bacteria in the natural environment,and to apply them in remediation of PAH contaminated soil in northern cold area,a cold resistant bacterium,which could use humic acid (HA) adsorbed PAHs as sole carbon and energy sources was screened from freezing-thawing soils of Shenfu Irrigation Area.This bacterium was identified as Pseudomonas sp.SDR4 with physiological-biochemical characterization and 16S rDNA sequencing analyses.its degradation ability for of HA adsorbed PAHs was studied.The results showed that this Pseudomonas sp.SDR4 can degrade phenanthrene,pyrene,and benzo(a) pyrene in the soil with 73.88％,88％ and 49.39％ of efficiency respectively.Addition of HA enhanced biodegradation rate of phenanthrene,pyrene and benzo(a) pyreneby 15.9％,13.8％ and 8.6％ respectively.The enhancements of biodegradation occurred at the early stage of HA addition,especially in the first week.But were not so signficant in the later stage.If Pseudormonas sp.SDR4 is selected in the cold region as a immobilised strain,addition of HA can improve its remediation efficiency.This study provides new immobilized strain for remediation of PAH contaminated soil in northern cold area.
soil in the freezing and thawing processadsorbed PAHslow temperature microorganismsscreening
NETL
NSTL
万方数据
维普
