Changes of microbial communities in Suaeda rhizosphere soils in response to petroleum stress
Suaeda is a typical pioneer halophyte with significant potential applications in remediating contaminated coastal wetlands.To investigate the effects of petroleum pollution stress on Suaeda and its rhizosphere soil microbial community,experimental groups were established with low(L),medium(M),and high(H)levels of petroleum pollution,alongside a control group(BC)without adding petroleum.High-throughput sequencing of 16S rRNA gene amplicons was employed to analyze the soil microbial community.The results indicated that petroleum pollution significantly inhibited Suaeda growth.The pH and total phosphorus levels in rhizosphere soils were notably affected by petroleum concentration.Petroleum pollution promoted the secretion of extracellular polysaccharides in the soil but had no significant impact on extracellular proteins and soil enzyme activities.Additionally,petroleum pollution reduced the diversity of soil microbial communities and induced changes in community structure.The dominant phyla in experimental groups were Proteobacteria,Bacteroidota,Actinobacteriota and Firmicutes,while the dominant genera were Marinobacter,Methylophaga,Alcanivorax,Pseudomonas and Kangiella.The relative abundances of these dominant genera greatly increased under petroleum pollution stress.Moreover,petroleum pollution significantly affected the abundances of petroleum degradation-related genes in Suaeda rhizosphere soils.This study demonstrated that petroleum pollution stress altered the physicochemical properties and microbial community structure of Suaeda rhizosphere soils.These findings provide a scientific basis for understanding the impact of petroleum pollution on coastal wetland ecosystems.
Suaedapetroleum pollutionhigh-throughput sequencingmicrobial community
NETL
NSTL
万方数据
