The effect of Aneurinibacillus on Ochromonas based on metabolomics analysis
The relationship between algae and bacteria is of great significance for microalgae culture systems,and constructing microalgal-bacterial symbiotic system to increase microalgae biomass production is an effective strategy for the application of microalgae resources.UHPLC-QTOFMS(Ultra high performance liquid chromatography quadrupole time of flight mass spectrometry)was adopted to reveal the effects of Aneurinibacillus on Ochromonas at the metabolic level in autotrophic and mixtrophic culture systems.The results demonstrated that the impact of Aneurinibacillus on the growth and metabolism of microalgae under autotrophic environment was mainly achieved through taurine and hypotaurine metabolism,valine,leucine and isoleucine biosynthesis,arginine biosynthesis,ascorbate and aldarate metabolism and galactose metabolism pathways.Under mixtrophic environment it was mainly achieved through valine,leucine and isoleucine biosynthesis,lysine degradation and TCA cycle.Glucose addition into the microalgae system with bacterial culture reduced the algal promoting effect of Aneurinibacillus with the expression levels of L-glutamate and L-glutamine significantly downregulated.The pathways of ABC transporters,aminoacyl-tRNA biosynthesis,nitrogen metabolism,glyoxylate and dicarboxylate metabolism,alanine,aspartate and glutamate metabolism and arginine biosynthesis were evidently affected.This study provides guidance for revealing the typical mechanism of algal and bacterial action and establishing an efficient symbiotic system.
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