摘要
藻菌关系对微藻培养体系具有重要意义,构建藻菌共生体系提高微藻生物质产量是微藻资源应用的重要策略.以棕鞭藻为研究对象,采用基于超高效液相色谱-四极杆飞行时间质谱联用技术揭示解硫胺素芽孢杆菌Aneurinibacillus对微藻在自养和混养培养体系中的代谢水平的影响.结果表明,Aneurinibacillus在无糖环境中对微藻生长代谢的影响主要通过牛磺酸和次牛磺酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、精氨酸生物合成、抗坏血酸和醛酸代谢及半乳糖代谢通路实现;在有糖环境中对微藻生长代谢的影响主要通过缬氨酸、亮氨酸和异亮氨酸生物合成、赖氨酸降解和TCA循环通路实现.有菌体系中,葡萄糖的加入会降低Aneurinibacillus的促藻效应,L-谷氨酸和L-谷氨酰胺表达量会显著下调,微藻细胞的ABC转运蛋白、氨酰-tRNA生物合成、氮代谢、乙醛酸盐和二羧酸盐代谢、丙氨酸、天冬氨酸和谷氨酸代谢、精氨酸生物合成均受到显著影响.本研究可为揭示典型藻菌作用机制和高效藻菌共生体系的建立提供指导.
Abstract
The relationship between algae and bacteria is of great significance for microalgae culture systems,and constructing microalgal-bacterial symbiotic system to increase microalgae biomass production is an effective strategy for the application of microalgae resources.UHPLC-QTOFMS(Ultra high performance liquid chromatography quadrupole time of flight mass spectrometry)was adopted to reveal the effects of Aneurinibacillus on Ochromonas at the metabolic level in autotrophic and mixtrophic culture systems.The results demonstrated that the impact of Aneurinibacillus on the growth and metabolism of microalgae under autotrophic environment was mainly achieved through taurine and hypotaurine metabolism,valine,leucine and isoleucine biosynthesis,arginine biosynthesis,ascorbate and aldarate metabolism and galactose metabolism pathways.Under mixtrophic environment it was mainly achieved through valine,leucine and isoleucine biosynthesis,lysine degradation and TCA cycle.Glucose addition into the microalgae system with bacterial culture reduced the algal promoting effect of Aneurinibacillus with the expression levels of L-glutamate and L-glutamine significantly downregulated.The pathways of ABC transporters,aminoacyl-tRNA biosynthesis,nitrogen metabolism,glyoxylate and dicarboxylate metabolism,alanine,aspartate and glutamate metabolism and arginine biosynthesis were evidently affected.This study provides guidance for revealing the typical mechanism of algal and bacterial action and establishing an efficient symbiotic system.
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