Study on Biodiversity and Community of Diatom in Caohai Lake Based on eDNA Metagenomics
The emerging technology of environmental DNA(eDNA)has significant advantages for biodiversity monitoring,including non-invasiveness,high efficiency,and sensitivity.To assess the adaptability of eDNA technology based on metagenomic sequencing for diatom monitoring in karst lakes,water samples and surface sediment samples were collected from the lakeside zone of Caohai in Guizhou Province,and the community composition,biodiversity and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway of planktic and sedimentary diatoms were analyzed by combining metagenomics with eDNA.The results showed that:(1)The Bacillariophyta in Caohai wetland consisted of 4 classes,23 orders,36 families,54 genera,and 78 species.In terms of the taxonomic family,Naviculaceae and Thalassiosiraceae were identified as the dominant groups.The average Chao1 index of the diatom community was determined to be 42.88±15.35,and the average Shannon-Wiener index was 2.09±0.29.(2)The global and overview maps were found to be the most representative functions among the KEGG pathway analyzed,followed by energy metabolism and translation pathways.The dominant KO genes included the atpF gene,secA gene,rplT gene,rpoA gene,and argH gene.(3)Principal coordinate analysis(PCoA)along with analysis of similarities(ANOSIM)revealed significant differences between environmental media(water and surface sediment)in terms of diatom communities.The Linear discriminant analysis Effect Size(LEfSe)analysis revealed that the different biomarkers characterized planktonic diatom communities mainly by Thalassiosira and Cyclotella,while the sedimentary diatoms were primarily represented by Fistulifera,Phaeodactylum,and Nanofrustulum.Wilcoxon rank-sum test showed that the differential genes of planktic diatoms were concentrated in the folate biosynthesis pathway and pyrimidine metabolism,while those of sedimentary diatoms were concentrated in photosynthesis,oxidative phosphorylation,and other metabolic functions.The research indicates that highly sensitive eDNA metagenomic technology can effectively describe the diatom biodiversity in Caohai Lake,thereby demonstrating its significant potential for application in lake biodiversity monitoring and water ecological environmental health assessment.
diatom communityplateau wetlandmetagenomicsenvironmental DNA
NETL
NSTL
万方数据
维普
