首页|Purple acid phosphatase promoted hydrolysis of organophosphate pesticides in microalgae

Purple acid phosphatase promoted hydrolysis of organophosphate pesticides in microalgae

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When organophosphate pesticides(OPs)are not used and handled in accordance with the current rules and standards,it results in serious threats to the aquatic environment and human health.Phaeodactylum tricornutum is a prospective microalgae-based system for pollutant removal and carbon sequestration.Genetically engineered P.tricornutum,designated as the OE line(endogenously expressing purple acid phosphatase 1[PAP1]),can utilize organic phosphorus for cellular metabolism.However,the compe-tencies and mechanisms of the microalgae-based system(namely the OE line of P.tricornutum)for metabolizing OPs remain to be addressed.In this study,the OE line exhibited the effective biodegra-dation competencies of 72.12％and 68.2％for 30 mg L-1 of dichlorvos and 50 mg L-1 of glyphosate,accompanied by synergistic accumulations of biomass(0.91 and 0.95 g L-1)and lipids(32.71％and 32.08％),respectively.Furthermore,the biodiesel properties of the lipids from the OE line manifested a high potential as an alternative feedstock for microalgae-based biofuel production.A plausible mecha-nism of OPs biodegraded by overexpressed PAP1 is that sufficient inorganic P for adenosine triphosphate and concurrent carbon flux for the reduced form of nicotinamide adenine dinucleotide phosphate biosynthesis,which improved the OP tolerance and biodegradation competencies by regulating the antioxidant system,delaying programmed cell death and accumulating lipids via the upregulation of related genes.To sum up,this study demonstrates a potential strategy using a genetically engineered strain of P.tricornutum to remove high concentrations of OPs with the simultaneous production of biomass and biofuels,which might provide novel insights for microalgae-based pollutant biodegradation.

BiodegradationLipid accumulationOrganophosphate pesticidePhaeodactylum tricornutumPurple acid phosphatase

Marco Zeppilli、Hafsa Yaqoubi、Edoardo Dell'Armi、Agnese Lai、Mustapha Belfaquir、Laura Lorini、Marco Petrangeli Papini、Wei-Dong Yang、Yalei Zhang、Hong-Ye Li

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Department of Chemistry,University of Rome Sapienza,Piazzale Aldo Moro 5,Rome,00185,Italy

Department of Chemistry,Ibn Tofail University,Laboratory of Advanced Material and Process Engineering,Campus Universitaire,BP.242,Kenitra,Morocco

Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes,College of Life Science and Technology,Jinan University,Guangzhou,510632,China

State Key Laboratory of Pollution Control and Resources Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai,200092,China

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国家自然科学基金国家自然科学基金Guangdong Basic and Applied Basic Research Foundation中国博士后科学基金中国博士后科学基金Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)

51908244318700272023A15150123142018M6433632019T120789SMSEGL20SC02

2024

10.1016/j.ese.2023.100318

环境科学与生态技术(英文)

环境科学与生态技术(英文)

ISSN：
年,卷(期)：2024.18(1)
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