Correlation between microbial community and environmental factors in new pollutant dichloromethane contaminated sites
It is of great practical significance for bioremediation of dichloromethane(DCM)contaminated sites to clarify the relationship between environmental factors,pollutant content levels and microbial communities.Taking a DCM contaminated plot as the research object,groundwater samples at different depths of 5.5～13.5 m were collected to determine their physicochemical properties and DCM concentration.The abundance,diversity,and species structure of microorganisms in the contaminated groundwater were analyzed through high-throughput sequencing.The results indicate that:1)Groundwater is in a weak oxidation and reduction environment,with a relatively high concentration of TOC,which is related to the high concentration of DCM.The content of SO2-4 and Cl-is relatively high,and the concentration range of TN,TP,and NO-3 is5.7～18.2,0.6～2.5 and2.7～17.2 mg/L,respectively;2)The vertical distribution of DCM pollution in groundwater is continuous,with pollution plumes mainly concentrated in the southwest of the plot.The volume of contaminated groundwater is 38 241.83 m3,and the concentration of DCM pollution gradually decreases with depth,with a maximum exceeding depth of 13.5 m.The maximum pollution depth is located in the muddy clay layer;3)The diversity of microbial communities is significantly negatively correlated with the concentration of DCM pollutants.DCM pollution generally inhibits the growth and reproduction of microorganisms,while TOC,TN,and TP are significantly positively correlated with microbial community diversity.TOC,TN and TP can significantly affect the richness of microbial species;4)Pseudomonadota and Bacillota are the dominant bacteria in groundwater.The main dominant bacteria are Pseudomonas,Hyphomicrobium,Nocardioides and Methylobacterium.Among them,TN and TP are significantly positively correlated with Methylobacterium,DCM and Cl-concentrations are significantly negatively correlated with Methylobacterium,and DCM concentrations are significantly negatively correlated with Pseudomonas.The research results provide a reference for the research and development of bioremediation technology for DCM contaminated land.
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