In this paper,the agile transceiver AD9361 RF chip and serial communication were introduced;due to the fast and portable features of AD9361 chip parameters configuration,combined with simple and reliable serial communication,it was proposed that the AD9361 RF chip frequency,power,bandwidth and other parameters can be change rapidly,without restarting the hardware board,PC sends configuration files to AD9361 RF chip through serial port.At last the configuration of AD9361 RF chip was realized on Xilinx FPGA by sending configuration file through serial port and on the basis of running software without interruption.