The impact of NCG supplementation on semen quality of large-bone chickens under heat stress
The aim of this study was to investigate the effects of dietary supplementation of different concentrations of N-carbamylglutamic acid(NCG)on semen quality of large-bone chickens under heat stress.Forty-eight 25-week-old large-bone chickens were randomly divided into four groups,in each of which was 12 chickens.The control group was fed the basal diet,and the experimental groups(N1,N2 and N3)were fed the basal diets supplemented with 0.08%,0.12%and 0.16%NCG,respectively.The motility,viability,malformation rate and antioxidant indexes of semen of big-bone chickens were tested weekly for a total of 90 d.The results showed as follows:compared with the control group,the semen vitality,progression and plasma membrane integrity were significantly increased(P<0.05),the rates of sperm malformation were significantly decreased(P<0.05),while the oscillating and whipping frequency showed no significant difference(P>0.05)in 3 experimental groups.The superoxide dismutase(SOD)levels in group N3 were significantly higher than those in group N1(P<0.05),and there was no significant difference(P>0.05)for CAT activity and the content of MDA among the groups.The study indicated that dietary supplementation of NCG could reduce the detrimental impact of heat stress to the semen quality of large-bone chickens and the supplementation of 0.16%NCG could produce the best mitigation effect.
NCGheat stresslarge-bone chickensemen qualityantioxidant index