冷应激对民猪肌肉组织中抗氧化相关基因表达的影响
Effect of cold stress on the expression of antioxidant-related genes of skeletal muscle in Min pig
王芳 1马红 2刘娣2
作者信息
- 1. 黑龙江省农业科学院畜牧研究所,哈尔滨 150086;黑龙江省农业科学院博士后科研工作站,哈尔滨 150086
- 2. 黑龙江省农业科学院畜牧研究所,哈尔滨 150086
- 折叠
摘要
为了明确冷应激对民猪肌肉组织抗氧化相关基因表达的影响,试验选取1～2月龄的民猪仔猪9头,随机分为3组,分别饲养在常温(22～25℃,对照)、短期低温(4 ℃ 3 d)和长期低温(4 ℃15 d)环境下.通过实时荧光定量PCR的方法检测常温组、短期低温组和长期低温组民猪仔猪的背最长肌中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)基因、核因子E2相关因子2(Nrf2)、Kelch样环氧氯丙烷相关蛋白-1(Keap1)mRNA表达量的变化.结果表明:与常温对照组相比,短期低温组和长期低温组民猪肌肉组织中SOD基因表达量变化不显著(P＞0.05),而GPX表达量显著升高(P＜0.05);短期低温组Nrf2基因表达量显著升高(P＜0.05),但是长期低温组降低;短期低温组Keap1基因表达量变化不显著(P＞0.05),而长期低温组中其表达量显著下降(P＜0.05).说明冷应激对民猪肌肉组织抗氧化物相关基因的表达有一定的影响.
Abstract
The aim of this experiment was to study the effect of cold stress on the expression of antioxidant-related genes in muscles of Min pigs.Nine Min piglets at 1-2 months were randomly divided into three groups,which were fed at room temperature(22～25 ℃),short-term cold exposure(4 ℃ for 3 d)and long-term cold exposure(4 ℃ for 15 d),respectively.The mRNA expression levels of superoxide dismutase(SOD),glutathione peroxidase(GPX),Nuclear factor erythroid 2-related factor 2(Nrf2)and Kelch-like ECH-associated protein1(Keap1)in longissimus dorsi muscle of piglets in room temperature group,short term cold exposure group and long term cold exposure group were detected by quantitative real-time PCR.The results showed as follows:Compared with the room temperature control group,no significant difference in the expression of the SOD gene.However,the significant increase in GPX expression(P＜0.05)was detected in the short-term cold exposure group and the long-term cold exposure group.The expression level of Nrf2 gene was significantly increased in the short-term cold exposure group(P＜0.05)and decreased in the long-term cold exposure group.There was no significant difference in Keap1 gene expression between short-term cold exposure group and room temperature group,but it was significantly decreased in long-term cold exposure group(P＜0.05).The results indicated that cold stress could impact the expression of antioxidant-related genes in muscles of Min pigs.
关键词
冷应激/民猪/肌肉/抗氧化相关基因/qRT-PCRKey words
cold stress/Min pig/muscle/antioxidant-related genes/quantitative real-time PCR引用本文复制引用
基金项目
国家生猪技术创新中心先导项目(NCTIP-XD1C16)
国家自然科学基金项目(U20A2052)
黑龙江省省属科研院所科研业务费项目(CZKYF2023-1-C004)
出版年
2024
国家科技期刊平台
NSTL
万方数据