首页|影响羊供体超数排卵与受体移植受胎率的因素分析

影响羊供体超数排卵与受体移植受胎率的因素分析

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为了研究影响羊供体超数排卵效果及胚胎移植受体受胎率的因素,试验选取萨福克羊和东弗里升羊2个供体品种,受体羊为本地阿勒泰羊,对促卵泡素(FSH)不同批次、长途运输应激因素、不同输精方式、技术员的熟练程度以及受体同期化时间差等5个方面的影响因素开展了相关研究.结果表明:FSH的不同批次对萨福克和东弗里升2种供体羊可用胚率无显著影响(P＞0.05);长途运输组供体羊,无论是萨福克羊还是东弗里升羊,胚胎数和受胎率均极显著低于对照组(P＜0.01);不同输精方式,子宫角+常规输精的获胚率极显著高于常规输精、子宫角输精(P＜0.01),子宫角输精显著高于常规输精(P＜0.05);胚胎移植操作熟练组受胎率显著高于对照组(P＜0.05);受体羊撤栓时间不同,发情时间相差±12 h,两组受体羊受胎率均无显著差异(P＞0.05).本试验结果可为从事胚胎移植工作的科研工作者和技术员提供借鉴.
Analysis on the factors affecting the superovulation effects of donors and the pregnancy rate of recipients in sheep
In order to study the factors affecting the effect of superovulation of donors and the pregnancy rate of recipients in embryo transplantation of sheep,two sheep breeds,Suffolk sheep and East fryson sheep,were treated as donors,and local Altay sheep breeds were as recipients.The five factors including the batches of follicle-stimulating hormone(FSH),long-distance transportation,insemination methods,technician's skills and recipients'synchronization time were studied in the experiment.The results showed as follows:no significant difference was found for the effect of the batches of FSH on the available embryo rate of Suffolk and East fryson sheep(P＞0.05).A significant decrease in the embryo number and fertilization rate of the donor sheep,both Suffolk sheep and East fryson sheep after long-distance transportation,compared with that of the control group(P＜0.01).The insemination method of uterine horn insemination+conventional insemination could produce a higher recovery rate of embryos than that of either conventional or horn fertilization(P＜0.01),respectively,in which the recovery rate of uterine horn insemination was significantly higher than that of conventional insemination method(P＜0.05).The pregnancy rate of recipients group by skilled operators significantly higher than that of control group(P＜0.05).No significant difference in both estrus rate and conception rate between the recipients was found with±12-hours difference in onsets of estrus by different device removal time(P＞0.05).The results of the experiment could provide a reference for scientific researchers and technicians engaged in embryo transfer work.

sheepdonorrecipientsuperovulationovulation effectpregnancy rate

张杰、曾凡明、刘长彬、倪建宏、张振良、卢守亮、万鹏程、刘昱成、吕占民、刘伟俊

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新疆生产建设兵团第十师一八八团农业发展服务中心,新疆北屯 832006

新疆联远农牧科技发展有限公司,新疆伊宁 835000

省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子 832000

石河子大学生命科学学院,新疆石河子 832000

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羊 供体 受体 超数排卵 排卵效果 受胎率

新疆维吾尔自治区乡村振兴项目新疆生产建设兵团农业GG项目贵州大学高原山地动物遗传育种与繁殖教育部重点实验室开放课题项目新疆生产建设兵团科技合作计划项目新疆生产建设兵团科技创新人才计划项目中央引导地方科技发展资金项目

2022NC099黔教合KY字[2022]370号2023AB009-012020CB022

2024

10.19848/j.cnki.ISSN1005-2739.2024.01.0012

黑龙江动物繁殖
黑龙江省家畜繁育指导站

黑龙江动物繁殖

影响因子：0.134
ISSN：1005-2739
年,卷(期)：2024.32(2)
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