Screening genes related to laying traits of Zi geese based on whole genome re-sequencing
The aim of this study was to screen the key regulatory genes related to egg production traits in Zi geese.Based on the laying records,blood samples of 30 high-production and 30 low-production 300-day-old Zi geese were collected for genomic DNA extraction and whole genome re-sequencing.The whole genome re-sequencing results were used to screen differential SNPs and annotate genes.After performing the Fixation index(Fst)for signal analysis,GO function and KEGG pathway enrichment analyses were conducted to screen for candidate genes associated with the egg-laying traits of geese.The laying rate of high-production Zi geese was significantly higher than that of low-production during 2 to 15 weeks(P<0.01).Whole genome re-sequencing yielded 1 556.48 Gb(n=60)data with an average depth of 21.62,and the the average coverage rate was 98.78%.High and low production Zi geese were screened for 12 187 636 and 12 291 795 SNPs,respectively,with the variations mainly located in introns and non-coding regions.A total of 829 candidate genes were identified by Fst selection signature,and MAPK9,TGFB2 and SGPL1 genes were associated with egg production trait by GO function and KEGG pathway enrichment analysis.The results indicated that the three selected candidate genes had influence on laying traits in Zi geese.
Zi geesewhole-genome re-sequencingegg production traitsselection signaturecandidate genes
NETL
NSTL
万方数据
