The influence of potassium ionophore valinomycin on the sperm acrosome reaction of wapiti
The study of the sperm acrosome reaction is one of the most fundamental fields in fertilization biology.At present,the wapiti(Cervus elaphus)is one of the economically specialized animals raised in China.However,the exploration of the biological mechanisms of in vitro fertilization in wapiti are less reported both domestically and internationally.The aim of this study was to investigate the impact of valinomycin(Val)on the acrosome reaction,the optimal reaction system and ion concentration changes through capacitating wapiti sperm by using the float-up method and inducing the sperm acrosome reaction with Val.This research intended to provide scientific reference for improving the in vitro culture and in vitro fertilization efficiency of sperm and offer foundational data for the exploration of the fertilization mechanisms in cervids.The results suggested that Val at concentrations of 0.01 to 50.00 μmol/L could enhance the acrosome reaction rate of wapiti sperm.The optimal reaction system was to treat wapiti sperm with 0.50 μmol/L Val for 5.0 min,which achieved an acrosome reaction rate of(74.48±0.79)%,showing a significant difference from that of(33.44±3.47)%of Val-induced non-capacitated wapiti sperm(P<0.05).Moreover,the concentrations of Ca2+and K+in the culture medium varied during the acrosome reaction induced by Val.This study implied that Val could promote the sperm acrosome reaction in wapiti and possess the function of ion transport regulation.
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