The efficient proliferation and differentiation of myoblasts are crucial for the production of livestock meat products.In this experiment,the specific inhibitor OSMI-1 was employed to inhibit O-linked β-N-acetylglucosamine(O-GlcNAc)glycosylation to observe the changes in the cell cycle and differentiation of the mouse myoblast cell line(C2C12).Mouse C2C12 cells were used as experimental subjects,with a blank group,a DMSO group,and an OSMI-1/DMSO group established.Western Blot was utilized to determine the inhibition of O-GlcNAc modification by OSMI-1;flow cytometry was used to detect changes in the cell cycle;immunofluorescence was used to detect the differentiation marker(myosin heavy chain,Myhc),and qRT-PCR was used to detect the expression levels of differentiation factors(myogenic differentiation,Myod)and Myhc genes,to analyze the effect of O-GlcNAc on C2C12 myotube differentiation.The results indicated that the O-GlcNAc modification level of C2C12 was significantly inhibited by the OSMI-1/DMSO group at24 h and 48 h,and the proportion of G2 cells in the cell cycle was significantly reduced(P<0.05).During the differentiation process,the inhibition of O-GlcNAc modification resulted in a decrease in the expression of the differentiation marker Myhc,as well as a decrease in the expression of Myod and Myhc.
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