The effect of low temperature vitrification on the quality of fine tubes of Liaoning cashmere goat semen
Cooling balance is an important technical step in the production of Liaoning cashmere goat frozen semen.In order to improve the quality of Liaoning cashmere goat fine tube frozen semen,the temperature range of cooling balance was set to-1 to-2℃,and this low temperature cooling balance method was used to prepare fine tube frozen essence(experimental group).The quality of fine tube frozen sperm(0℃,control group)was compared with that obtained by conventional cooling balance method,including original sperm vitality,pre-freezing vitality,post-freezing vitality,survival time at 37℃ after freezing,and malformed sperm rate.Ewes aged 3 to 5 years old,1.5 to 2.5 years old and 10 to 12 months old were selected and divided into control groups and experimental groups.The fine tubes frozen semen of experimental groups 1,2 and 3 were obtained by low temperature cooling balance method,and the fine tube frozen semen of control groups 1,2 and 3 was obtained by conventional cooling balance method.The estrus conception rate between experimental group 1 and control group 1,experimental group 2 and control group 2,experimental group 3 and control group 3 were compared respectively.The results showed as follows:There were no significant differences between the experimental group and the control group in prosperm viability,pre-freezing viability and sperm malformation rate(P>0.05).The post-freezing viability and survival time at 37℃ of the experimental group were significantly higher than those of the control group(P<0.05).The impregnation rate of estrus in experimental groups were higher than that in control groups,and that in experimental group 1 was significantly higher than that in control group 1(P<0.05).This shows that the temperature of cooling equilibrium can be set to-1 to-2℃ when making Liaoning cashmere goat fine tube frozen essence,which is conducive to improving the quality of fine tube frozen essence and the transmission and delivery rate.
cashmere goatfine tube frozen semensub zero low temperaturecooling balancequality of frozen semen
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