The effects of feeding methods on the gut microbiota and blood biochemical indicators of fattening cattle
To investigate the effects of free-scattered and tethered feeding methods on the composition of intestinal microbiota,serum biochemical,and antioxidant indicators in fattening cattle,30 healthy Simmental hybrid cattle with adequate body condition and similar body weight(average body weight of[375.40±45.01]kg)were randomly divided into a free-scattered group and a tethered group,each with 15 cattle.The experiment was conducted for a pre-test period of 10 d and a formal period of 60 d,and fresh fecal and blood samples were collected from two groups to determine the composition of gut microbiota and serum biochemical and antioxidant indicators at the day of 60.The results showed that the relative abundance of phylum of Firmicutes,Bacteroidetes,Spirobacteria and Patellaceae was greater than 1%in both groups,with the Firmicutes and Bacteroidetes predominating.The relative abundance of Firmicutes(P<0.05),serum high-density lipoprotein cholesterol(HDL)levels(P<0.05)and serum glucose(GLU)levels(P<0.01)in the free-scattered group were significantly higher,while the relative abundance of Bacteroidetes(P<0.01),creatinine(CREA)levels were significantly lower than in the tethered group.In conclusion,the free-scattered feeding method could increase the relative abundance of Firmicutes and decrease the abundance of Bacteroidetes in the intestinal tract of cattle during the fattening period,which would be beneficial for the growth and development of fattening cattle,compared with the tethered feeding method.