首页|融合魏斯氏菌P2胞外多糖对巨噬细胞RAW264.7增殖及免疫调节活性的影响

融合魏斯氏菌P2胞外多糖对巨噬细胞RAW264.7增殖及免疫调节活性的影响

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以乳酸菌融合魏斯氏菌(Weissella confusa,W.confusa)P2为出发菌株,通过去菌体、去蛋白、乙醇沉淀和凝胶过滤层析等步骤从发酵液中获得纯胞外多糖(Exopolysaccharides,EPS),并以巨噬细胞RAW264.7为模型,采用CCK-8(Cell counting kit-8)法、中性红实验和细胞因子试剂盒探究W.confusa P2 EPS的体外免疫调节活性.结果表明,W.confusa P2 EPS可以显著促进巨噬细胞的增殖,提升巨噬细胞的吞噬能力和释放NO的能力,并在生物量水平上提高巨噬细胞中白介素-1β(Interleukin-1 β,IL-1β)、白介素-8(Interleukin-8,IL-8)和白介素-10(Interleukin-10,IL-10)细胞因子的含量,但不能提高单核细胞趋化蛋白-1(Monocyte chemotactic protein-1,MCP-1)和白介素-6(Interleukin-6,IL-6)细胞因子的含量,说明W.confusa P2 EPS具有良好的免疫调节能力.本研究结果可为W.confusa P2 EPS的构效关系研究和免疫产品开发提供理论基础.
Effect of exopolysaccharides from Weissella confusa P2 on the proliferation and immunomodulatory activity of macrophage RAW264.7
Lactic acid bacteria Weissella confusa(W.confusa)P2 was used as the starting strain,and the pure exopolysaccharides(EPS)was obtained from the fermentation solution through steps of debacterialization,deproteinization,ethanol precipitation and gel filtration chromatography.Using macrophages RAW264.7 as the model,cell counting kit-8,neutral red test and cytokine kit were used to explore the immunomodulatory activity of EPS in vitro.The results show that EPS can significantly promote the proliferation of macrophages,increase the phagocytosis ability of macrophages and the ability to release NO,and increase the contents of interleukin-1β(IL-1 β),interleukin-8(IL-8)and interleukin-10(IL-10),but can not increase the contents of monocyte chemotactic protein-1(MCP-1)and interleukin-6(IL-6)cytokines in macrophages RAW264.7 at the biomass level.W.confusa P2 EPS has a good ability of immune regulation.The results can provide a theoretical basis for the study of the structure-activity relationship of EPS and the development of immune products.

Weissella confusaexopolysaccharidesRAW264.7proliferationimmune activity

赵丹、赵守祺、王烁、陈曦、杜仁鹏

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黑龙江大学生命科学学院农业微生物技术教育部工程研究中心/黑龙江省寒区植物基因与生物发酵重点实验室/黑龙江省普通高校微生物重点实验室,哈尔滨 150080

河北环境工程学院河北省农业生态安全重点实验室,秦皇岛 066102

融合魏斯氏菌 胞外多糖 RAW264.7 增殖 免疫活性

黑龙江省自然科学基金优秀青年基金中国博士后科学基金黑龙江省博士后科学基金新时代龙江优秀硕士、博士学位论文资助项目河北省农业生态安全重点实验室开放基金黑龙江省省属高校基本科研业务费专项

YQ2021C0302022MD713755LBH-Z1082LJYXL2022-0202023SYSJJ172022-KYYWF-1075

2024

10.13482/j.issn1001-7011.2024.03.106

黑龙江大学自然科学学报
黑龙江大学

黑龙江大学自然科学学报

CSTPCD
影响因子：0.27
ISSN：1001-7011
年,卷(期)：2024.41(2)
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