Transcriptome sequencing analysis of Komagataeibacter hansenii HDM1-3 at different fermentation time
To investigate the differential gene expression levels of Komagataeibacter hansenii HDM1-3 at different fermentation times,and explore the function of differentially expressed genes in bacterial cellulose(BC)bioanabolic genes,Illumina HiSeq platform was used to analyze the transcriptome sequencing of Komagataeibacter hansenii HDM1-3 strain at the early stage(18 h)and the late stage(36 h)of fermentation,and fluorescence quantitative PCR was used to verify the accuracy of transcriptome sequencing results.Differentially expressed genes were analyzed by GO enrichment and KEGG enrichment.A total of 3154 CDS sequences are obtained by transcript sequencing,among which 57 sequences are not annotated,with an annotation rate of 98.22％.Differentially expressed genes analysis shows that there are 684 differentially expressed genes in strain HDM1-3 after 36 h fermentation compared with 18 h fermentation,among which 369(53.9％)genes are up-regulated and 315(46.1％)genes are down-regulated.GO functional enrichment analysis shows that the functional genes related to carbohydrate metabolism are down-regulated,while the functional genes related to cell membrane are up-regulated in the late fermentation stage.KEGG enrichment analysis shows that major energy metabolism pathways,including glycolysis pathway,pentose phosphate pathway and citric acid cycle,are significantly down-regulated,and two BC synthase genes are significantly up-regulated.These results provide theoretical reference for further analysis of BC synthesis-related genes in HDM1-3 strain to improve BC yield.
NETL
NSTL
万方数据
