摘要
以非转基因型大豆品种黑农48(病害敏感型的高蛋白品种)作为试验材料,以根内根孢囊霉(Rhizophagus intraradices,R.intraradices)为试验菌剂分别接种于大豆连作1年和非连作的大田土壤中,采用碱解离-酸性品红染色法和形态观测法测定根内根孢囊霉对连作苗期大豆根系丛枝菌根(Arbuscular mycorrhizal,AM)真菌侵染率、植株株高及茎粗的影响.结果显示,未施加根内根孢囊霉菌剂的大豆,连作导致AM真菌侵染率增高,株高和茎粗降低.而接种根内根孢囊霉的大豆,AM真菌侵染率、株高和茎粗均显著提高,表明根内根孢囊霉对连作大豆苗期植株生长发育具有一定促进作用.本研究为AM真菌的田间应用及提升黑龙江绿色大豆生产地位提供了理论依据.
Abstract
Non-transgenic soybean cultivar Heinong 48(a disease-sensitive high-protein variety)was used as experimental material.Rhizophagus intraradices(R.intraradices)was inoculated into soybean soil under the continuous cropping for 1 year and non-continuous cropping.The effect of R.intraradices on the colonization rate of arbuscular mycorrhizal(AM)fungi was determined by alkali dissociation-acid fuchsin staining.The effects of R.intraradices on the plant height and stem diameter of soybean roots at the seedling stage of continuous cropping were determined.The results show that the colonization rate of AM fungi increases and the plant height and stem diameter decrease in soybean with non-inoculated R.intraradices.However,the colonization rate of AM fungi,plant height and stem diameter of soybean inoculated with R.intraradices significantly increase,indicating that R.intraradices can promote the growth and development of soybean at the seedling stage under continuous cropping.A theoretical basis for the field application of AM fungi and the improvement of green soybean production in Heilongjiang is provided.
基金项目
黑龙江省自然科学基金资助项目(LH2023C087)
黑龙江省生态环境保护科研项目(HST2022TR001)
黑龙江省省属高等学校基本科研业务费项目(2022-KYYWF-1119)
哈尔滨市科技计划自筹经费资助项目(ZC2022ZJ002008)
NETL
NSTL
万方数据