Effect of Rhizophagus intraradices on plant growth of continuous cropping soybean at seedling stage
Non-transgenic soybean cultivar Heinong 48(a disease-sensitive high-protein variety)was used as experimental material.Rhizophagus intraradices(R.intraradices)was inoculated into soybean soil under the continuous cropping for 1 year and non-continuous cropping.The effect of R.intraradices on the colonization rate of arbuscular mycorrhizal(AM)fungi was determined by alkali dissociation-acid fuchsin staining.The effects of R.intraradices on the plant height and stem diameter of soybean roots at the seedling stage of continuous cropping were determined.The results show that the colonization rate of AM fungi increases and the plant height and stem diameter decrease in soybean with non-inoculated R.intraradices.However,the colonization rate of AM fungi,plant height and stem diameter of soybean inoculated with R.intraradices significantly increase,indicating that R.intraradices can promote the growth and development of soybean at the seedling stage under continuous cropping.A theoretical basis for the field application of AM fungi and the improvement of green soybean production in Heilongjiang is provided.
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