Isolution,identification and biological characteristics of a wild strain of Laetiporus cremeiporus
A wild mushroomfruiting body was collected from the campus of Northeast Forestry University.After the isolation and purification,based on the ITS sequence,a phylogenetic tree was established by molecular biological analysis,and the fungus was identified as Laetiporus cremeiporus(L.cremeiporus).The effects of different carbon sources,nitrogen sources,pH,and temperatures on the growth of mycelia were studied by single factor experiments.The optimal carbon,nitrogen,pH,and temperature for the mycelial growth of L.cremeiporus are glucose,(NH4)2SO4,4.0,and 20 ℃,respectively.Orthogonal experiments were designed and carried out according to the results of single factor experiments.The results show that the optimal factor composition for the mycelial growth of L.cremeiporus is glucose,beef extract,pH 4.0,and 30 ℃.The degree of various influence factors on mycelial growth is pH＞temperature＞carbon source＞nitrogen source.
Laetiporus cremeiporusITS sequence analysisbiological characteristicsoptimization of culture condition
NETL
NSTL
万方数据
