This article conducts UMNSAH/DF-1 cell culture experiments under different temperature condi-tions,while simulating the normal temperature transportation environment of live cell lines to find methods to improve the growth status and vacuolization phenomenon of UMNSAH/DF-1 cells.The experimental results indi-cate that compared to 37 ℃,a culture temperature of 39 ℃ is more conducive to the growth of UMNSAH/DF-1 cells.The degree of vacuolization of UMNSAH/DF-1 cells is significant during transportation at room tempera-ture.However,after normal passage,the phenomenon of cellular vacuolization will be significantly reduced.This article suggests that if there are many vacuoles in the cell body of UMNSAH/DF-1 cells,improvement can be achieved by optimizing the optimal growth temperature and increasing the number of passages.Generally,after multiple passages,UMNSAH/DF-1 cells will return to their normal state.
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维普
